Recently, mechanism of HSP20 and HSP90 families were studied to understand of heat response system in soybean ( Lopes-Caitar et al. In soybean, heat stress significantly decreased plant growth and yield. Soybean is one of important crop plant in worldwide. 2014) and regulated genes in Brassica juncea under drought stress and high temperature ( Bhardwaj et al. 2014), HD-Zip transcription factors in soybean under salt stress ( Belamkar et al. It was also used to reveal differential expressed transcriptome in rice under cold conditions ( Shen et al. 2009 Anders and Huber 2010 Rapaport et al. Because of the low cost and high capacity of data, RNA-sequencing (RNA-seq) has been using to measuring differentially expressed genes (DEGs) in response to stress ( Marioni et al. Since the development of next generation sequencing method, gene and transcript analysis has made possible with quickly and cost effectively. 2012) and expression gene profiles of HSPs and HSFs were identified using microarray analysis and quantitative real time PCR ( Hu et al. Some studies had tried to reveal a function of HSPs, HSFs and chaperones from Zea mays, rice, Arabiodopsis and soybean ( Queitsch et al. These families also have been characterized in a few plants ( Krishna and Gloor 2001 Hu et al. Plant HSPs are classified into some types according to molecular size ( Sarkar et al. Also, these stress response systems are regulated by HSFs which has DNA binding sites ( Schöffl et al. Chaperones and HSPs have functions which restoration of mis-folding proteins and minimizing damages for maintenance plant homeostasis ( Boston et al. These damaged proteins are interfering to normal cell functioning. Heat stress has make mis-folded proteins and cause to losing of biological activity in plant. 2007 Larkindale and Vierling 2007 Yamada et al. Since the HSPs expression observed under heat stress ( Lindquist and Craig 1988), the heat stress response pathway has been continually studied in plant ( Queitsch et al. Among many stress response systems, heat shock proteins (HSPs), heat shock transcription factors (HSFs) and chaperone are essential for maintenance and restoration of protein structure and stabilized condition of plants under heat stress ( Boston et al. Plants could not move their position to avoid stresses, stress response system with molecular level is important to growth and productivity in plants. Among abiotic stress, heat stress is one of the factors disturb productivity and growth of plants. Global climate change is becoming a serious condition to plants. Keywords : Heat stress, RNA sequencing, Transcriptome, Expression pattern, Soybean The expression profiling and characterization provide invaluable information to understand heat tolerance of soybean. On the other hand, large molecule HSPs, HSFs and chaperonin did not response to long heat stress. As a result, relatively small HSP families were up regulated and continuously expressed in long period heat stress. Finally, we find 68 genes involved in HSP, HSF, chaperones in heat responsive genes associated increasing heat tolerance. We also revealed HSPs, HSFs and chaperones in the differentially expressed genes using BLAST and Pfam analyzation and verified expression changes under heat stress. The transcripts were analyzed, and we obtained 2,458 genes including 46 co-up regulation and 55 co-down regulated genes in both soybean varieties. To reveal responsive gene to heat stress, we performed RNA-seq using two Korean soybean varieties under heat stress and normal conditions. Many genes including heat shock protein (HSP), heat shock transcription factors (HSF) and chaperones, were identified and characterized in many plants to play role in increased tolerance to abiotic stress. Heat stress is one of the factors disturb productivity and growth of plants.
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